• 靶点-Target： MMP-9
• MW(Observed)： 66kD，78kD
• 宿主物种-Host Species： Rabbit
• 同种型-Isotype： IgG
• 修饰-Modification： Unmodified
• 推荐稀释比： WB 1:500-2000;IHC 1:50-300
• 组成： Liquid in PBS containing 50% glycerol， 0.5% BSA and 0.02% sodium azide.
• 纯化工艺： The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
• 储存： -15°C to -25°C/1 year(Do not lower than -25°C)
• 浓度： 1 mg/ml
• 克隆性： Polyclonal
• 免疫原： Synthesized peptide derived from human MMP9 (Cleaved-Phe107)
• 特异性： This antibody detects endogenous levels of Human MMP9 (Cleaved-Phe107， protein was cleaved amino acid sequence between 106-107 )
• 基因名称： MMP9 CLG4B
• 蛋白名称： MMP9 (Cleaved-Phe107)
• Organism-1： Human
• 基因ID-1： 4318
• SwissProt-1： P14780
• Organism-2： Mouse
• 基因ID-2： 17395
• SwissProt-2： P41245
• Organism-3： Rat
• 基因ID-3： 81687
• SwissProt-3： P50282
• 背景： catalytic activity:Cleavage of gelatin types I and V and collagen types IV and V.，cofactor:Binds 2 zinc ions per subunit.，cofactor:Binds 3 calcium ions per subunit.，disease:Defects in MMP9 may be a cause of susceptibility to lumbar disk herniation (LDH) [MIM:603932]. LDH is the predominant cause of low-back pain and unilateral leg pain.，domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion， thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.，enzyme regulation:Inhibited by histatin-3 1/24 (histatin-5).，function:May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.，induction:Activated by 4-aminophenylmercuric acetate and phorbol ester.，miscellaneous:In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.，PTM:N- and O-glycosylated.，PTM:Processing of the precursor yields different active forms of 64， 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.，similarity:Belongs to the peptidase M10A family.，similarity:Contains 3 fibronectin type-II domains.，similarity:Contains 4 hemopexin-like domains.，subunit:Exists as monomer， disulfide-linked homodimer， and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form.，tissue specificity:Produced by normal alveolar macrophages and granulocytes.，
• 细胞定位： Secreted， extracellular space， extracellular matrix .

资料下载