模式菌株	模式菌株
别名	NBRC113892=DSM108848
参考用途	科研
生物危害程度	四类
培养温度	30℃
需氧类型	好氧
提供方式	冻干粉
培养条件	VY/2 AGARBaker’s yeast 5.00 g Vitamin B12 0.50 mg CaCl2 x 2 H2O 1.36 g Agar (Difco) 15.00 g Distilled water 1000.00 ml Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension (5 g baker’s yeast/100 ml distilled water， adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before， and after autoclaving and cooling to 50°C (use pH-indicator paper). For suspension of freeze-dried cells from ampoules add about 0.5 - 1.0 ml medium MD1 (per liter: casiton 3.0 g; calciumchloride dihydrate 0.7 g; magnesiumsulphate heptahydrate 2.0 g) to the vial with freeze dried material. For DSM 11116 reduce amount of vitamin B12 to 0.05 mg/l.