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深红红螺菌
深红红螺菌
  • 商品货号:FHJZ82458
  • 活动价: ¥1400元
    市场价:¥1600元
    已售 0 件 | 评价 0 人次 | 关注度 72
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    模式菌株 模式菌株
    别名 DSM467=ATCC11170=NCIB8255
    参考用途 科研
    生物危害程度 四类
    培养温度 30℃
    需氧类型 厌氧,光照
    提供方式 冻干粉
    培养条件 红螺菌培养基酵母提取物 0.30 g 乙醇 0.50 ml 琥珀酸钠 1.00 g 乙酸铵 0.50 g 柠檬酸铁(0.1% in H2O) 5.00 ml KH2PO4 0.50 g MgSO4x 7 H2O 0.40 g NaCl  0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g 维生素B12 组份(10 mg in 100 ml H2O) 0.40 ml 微量元素组份 SL-6 (如下) 1.00 ml 蒸馏水  1050.00 ml pH 6.8. Boil the medium under a stream of nitrogen gas for few minutes and distribute 45 ml medium into 50 ml screw-capped bottles (already flushed with nitrogen gas). Bubble each bottle with nitrogen gas and close immediately with a rubber septum and screw tight. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. For brown and other oxygen sensitive Rhodospirillaceae add 300 mg of L-cysteine (0.03% end concentration) to the boiling medium and readjust the pH to 6.8 or to the prepared medium in bottles inject neutralized sulfide solution (0.005 to 0.01% end concentration). The medium has been modified according to reference 3365. 微量元素组份SL-6: ZnSO4 x 7 H2O  0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O  0.02 g Na2MoO4 x 2 H2O 0.03 g 蒸馏水 1000.00 ml