模式菌株 模式菌株 别名 =ATCC 35703 =CIP 103912 =DSM 11550 参考用途 模式菌株 生物危害程度 四类 培养温度 28℃ 提供方式 冻干物 保存温度 冷藏4-10℃ 培养条件 ANAEROCELLUM MEDIUM1059 ANAEROCELLUM MEDIUM酵母提取物 (Difco) 0.3g Na2-succinate 1.0g (NH4)-acetate 0.5g Fe(III) citrate solution (0.1% in H2O)5.0mlKH2PO4 0.5g MgSO4 x 7 H2O 0.4g NaCl 0.4g NH4Cl 0.4g CaCl2·2 H2O0.05g Vitamin B12 solution (10 mg in 100 ml H2O) 0.4mlg Trace element solution SL-10(见培养基1064)1.0ml L-Cysteiniumchloride 0.3g Resazurin(01%) 0.5 ml蒸馏水 1.0L Adjust pH to 6.8Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp.