模式菌株	模式菌株
别名	=DSM 19012
参考用途	模式菌株
生物危害程度	四类
培养温度	50℃
提供方式	冻干物
保存温度	冷藏4-10℃
培养条件	ANAEROBIC SEAWATER MEDIUM1063 ANAEROBIC SEAWATER MEDIUMSolution A:KH2PO40.2g NH4Cl0.25g NaCl20.0g MgCl2 . 6 H2O3.0gKCl0.5gCaCl2 . 2 H2O0.15g Na-resazurin solution (0.1% w/v)0.5ml蒸馏水 940.0ml Solution B:Trace element solution SL-101.0mlSolution C:Vitamin solution1.0mlSolution D:Selenite-tungstate solution1.0mlSolution E:NaHCO3 3.5g蒸馏水50.0mlSolution F:D-glucose2.0gYeast extract0.5g蒸馏水1.0LSolution G:Na2S·9H2O0.3g蒸馏水10.0mlSparge solution A with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B D and G are autoclaved separately under 100% N2 gas. Solutions C and F are prepared under 100% N2 gas and sterilized by filtration. Solution E is autoclaved under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.2 - 7.4 if necessary.Selenite-tungstate solution:NaOH0.5gNa2SeO3·5 H2O3.0mgNa2WO4·2 H2O4.0mg蒸馏水1.0LTrace element solution SL-10：HCl (25%; 7.7 M) 10.0ml FeCl2·4 H2O1.5gZnCl270.0mgMnCl2·4 H2O100.0mgH3BO36.0mgCoCl2·6 H2O190.0mgCuCl2 ·2 H2O2.0mgNiCl2·6H2O24.0mgNa2MoO4·2H2O36.0mg蒸馏水990.0mlFirst dissolve FeCl2 in the HCl then dilute in water add and dissolve the other salts. Finally make up to 1000.0 ml.Seven vitamins solution：Vitamin B12100.0mgp-Aminobenzoic acid80.0mgD(+)-Biotin20.0mgNicotinic acid200.0mgCalcium pantothenate100.0mgPyridoxine hydrochloride300.0mgThiamine-HCl x· 2 H2O200.0mg蒸馏水1.0L