- 商品货号:FH1840
- 规格:10μl质粒
- 原核抗性:卡那霉素Kan
- 克隆菌株:大肠杆菌DH5α
- 质粒图谱1: png/1-1P121135952Y8.png
质粒序列: 点击查看序列LOCUS Exported 5594 bp ds-DNA circular SYN 13-JAN-2018DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..5594/organism="synthetic DNA construct"/mol_type="other DNA"rep_origin 12..467/direction=RIGHT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"CDS complement(560..1375)/codon_start=1/gene="aph(3')-Ia"/product="aminoglycoside phosphotransferase"/label=KanR/note="confers resistance to kanamycin in bacteria or G418(Geneticin(R)) in eukaryotes"/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"rep_origin 1497..2085/direction=RIGHT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"misc_feature 2271..2413/label=bom/note="basis of mobility region from pBR322"CDS complement(2515..2706)/codon_start=1/gene="rop"/product="Rop protein, which maintains plasmids at low copynumber"/label=rop/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL"CDS complement(3515..4597)/codon_start=1/gene="lacI"/product="lac repressor"/label=lacI/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ"promoter complement(4598..4675)/gene="lacI"/label=lacI promoterpromoter 4984..5002/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"protein_bind 5003..5027/label=lac operator/bound_moiety="lac repressor encoded by lacI"/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."RBS 5042..5064/note="efficient ribosome binding site from bacteriophageT7 gene 10 (Olins and Rangwala, 1989)"CDS 5077..5094/codon_start=1/product="6xHis affinity tag"/label=6xHis/translation="HHHHHH"CDS 5095..5385/codon_start=1/gene="S. cerevisiae SMT3 (truncated)"/product="cleavable ubiquitin-like protein tag"/label=SUMO/translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG"CDS 5438..5455/codon_start=1/product="6xHis affinity tag"/label=6xHis/translation="HHHHHH"terminator 5522..5569/label=T7 terminator/note="transcription terminator for bacteriophage T7 RNApolymerase"ORIGIN1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa服务时间:24小时在线客服
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