- 商品货号:BR389
- 规格:10μl质粒
- 启动子:T7 promoter
- 复制子:pUC ori
- 质粒大小:2792bp
- 原核抗性:卡那霉素Kan
- 克隆菌株:大肠杆菌DH5α
- 质粒简介: pUC57-sgRNA质粒是一个用于体外转录sgRNA的载体,gRNA片段可通过BsaI酶切连入,并在T7启动子的驱动下转录出来。本质粒的参考文献是:Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.
- 质粒图谱1: png/1-1G026153934645.png
质粒序列: 点击查看序列LOCUS Exported 2792 bp ds-DNA circular SYN 26-OCT-2017DEFINITION synthetic circular DNASOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 2792)AUTHORS .TITLE Direct Submissionhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..2792/organism="synthetic DNA construct"/mol_type="other DNA"promoter 442..460/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"misc_RNA 482..557/label=gRNA scaffold/note="guide RNA scaffold for the Streptococcus pyogenesCRISPR/Cas9 system"rep_origin complement(1018..1606)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(1777..2592)/codon_start=1/gene="aph(3')-Ia"/product="aminoglycoside phosphotransferase"/label=KanR/note="confers resistance to kanamycin in bacteria or G418(Geneticin(R)) in eukaryotes"/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"promoter complement(2593..2697)/gene="bla"/label=AmpR promoterORIGIN1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa421 tgcatctaga tatcggatcc ctaatacgac tcactatagg tgagaccgag agagggtctc481 agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag541 tggcaccgag tcggtgcttt ttttaaaggg cccgtcgact gcagaggcct gcatgcaagc601 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca661 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa721 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag781 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgcggccgc841 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc901 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat961 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt1021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg1081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc1141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt1201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa1261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta1321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa1381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa1441 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt1501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt1561 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat1621 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat1681 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc1741 aatctaaagt atatatgagt aaacttggtc tgacagttag aaaaactcat cgagcatcaa1801 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt1861 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg1921 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat1981 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag2041 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc2101 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg2161 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc2221 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt2281 tttcccaggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt2341 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac2401 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc2461 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc2521 atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg2581 aatatggctc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct2641 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac2701 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta2761 taaaaatagg cgtatcacga ggccctttcg tc//
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